The distribution of free amino acids in subcellular fractions of guinea-pig brain
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چکیده
منابع مشابه
The distribution of free amino acids in subcellular fractions of guinea-pig brain.
1. The free amino acids of homogenates of guinea-pig brain in 0.32m-sucrose and of subcellular fractions derived therefrom have been estimated by the method of Moore & Stein. 2. Seven amino acids together accounted for over 80% of the free amino compounds; these are, in decreasing order of abundance: glutamate, aspartate, gamma-aminobutyrate, glycine, serine, alanine and threonine. In addition,...
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It is becoming increasingly apparent that lipids act as essential structural elements in the multienzyme systems associated with the cell organelles. Many of the enzymes of the electron-transport chain in mitochondria can be isolated as discrete lipoproteins (Green, 1959), and the phospholipid contained in these is essential for enzymic activity (Reich & Wainio, 1961). Moreover, lipids play a f...
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Gangliosides are acidic, acid-sensitive, nondialysable, water-soluble lipids containing Nacetylneuraminic acid, hexosamine, hexoses and ceramides which in the brain occur mainly in grey matter. The aim of the present investigation was to study the localization of gangliosides in the cerebral cortex by following their distribution in subcellular fractions, neuraminic acid content being used as m...
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1. The subcellular distributions of glutamate decarboxylase and aspartate transaminase were studied in rat and guinea-pig brain. 2. Glutamate decarboxylase is localized in the synaptosome fraction. The mean density of the particles containing the enzyme is slightly greater than those derived from cholinergic neurones, though overlap is substantial. 3. The enzyme is readily released from synapto...
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due to the limiting workspace of parallel manipulator and regarding to finding the trajectory planning of singularity free at workspace is difficult, so finding a best solution that can develop a technique to determine the singularity-free zones in the workspace of parallel manipulators is highly important. in this thesis a simple and new technique are presented to determine the maximal singula...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1966
ISSN: 0006-2936
DOI: 10.1042/bj0980128